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目的通过采用不同的动脉阻塞和阻塞时限建立大脑中动脉(MCA)远端阻塞(d MCAO)的脑缺血模型,比较脑梗死体积及行为学评分的差异,并确定最佳的阻塞动脉方式和阻塞时限。方法第1阶段:在相同阻塞时限(90 min)下,采用不同动脉阻塞方法建立脑缺血模型:1电凝一侧大脑MCA远端(MCAO组,n=10);2电凝MCA+同侧颈总动脉(CCA)阻塞90 min(MCAO+1CCAO*90组,n=10);3电凝MCA+双侧CCA阻塞90 min(MCAO+2CCAO*90组,n=10);另设对照组(MCA和CCA皆不阻塞,n=8)。造模24 h后检测脑梗死体积和行为学评分。第2阶段:采用电凝MCA+双侧CCA阻塞法,阻塞时限分别为30 min(MCAO+2CCAO*30组,n=10)、60 min(MCAO+2CCAO*60组,n=10)、120 min(MCAO+2CCAO*120组,n=10)建立脑缺血模型,于造模24 h后检测梗死体积和行为学评分。结果在相同阻塞时限(90 min)下,MCAO+2CCAO*90组的脑梗死体积和运动功能缺损较MCAO组及MCAO+1CCAO*90组明显,且差异有显著统计学意义(P<0.01)。MCAO组与MCAO+1CCAO*90组比较差异无统计学意义(P>0.05)。在阻塞相同动脉(电凝MCA+双侧CCA阻塞)条件下,MCAO+2CCAO*120组死亡率高达60%;MCAO+2CCAO*60组的脑梗死体积和运动功能缺损与MCAO+2CCAO*30组比较,差异有显著统计学意义(P<0.01)。MCAO+2CCAO*60组与MCAO+2CCAO*90组比较,差异无统计学意义(P>0.05)。结论电凝一侧MCA+60 min一过性阻塞双侧CCA制作d MCAO模型可产生相对明显的梗死体积和运动功能损伤,且死亡率低、造模时间短,适用于缺血性脑卒中研究。
OBJECTIVE: To establish a cerebral ischemia model of distal middle cerebral artery occlusion (MCA) using different arterial occlusion and occlusion time intervals, compare cerebral infarction volume and behavioral score, and determine the optimal mode of occlusion artery Obstruction time limit. Methods: The first phase of the method: under the same obstruction time (90 min), cerebral ischemia models were established by different arterial occlusion methods: 1 MCA distal to the side of electrocoagulation (MCAO group, n = 10); 2 MCA MCA + 1 CCAO * 90 group, n = 10); 3 MCA + bilateral CCA occlusion for 90 min (MCAO + 2 CCAO * 90 group, n = 10); another control group Neither MCA nor CCA blocked, n = 8). The cerebral infarction volume and behavioral score were measured 24 h after modeling. In the second stage, the MCA + bilateral CCA occlusion method was used. The occlusion time was 30 min (MCAO + 2 CCAO * 30 group, n = 10) (MCAO + 2CCAO * 120 group, n = 10). The infarct volume and behavioral score were measured 24 h after model establishment. Results Compared with MCAO group and MCAO + 1CCAO * 90 group, MCAO + 2CCAO * 90 group showed significant difference in infarct size and motor function under the same obstruction time (90 min) (P <0.01). There was no significant difference between MCAO group and MCAO + 1CCAO * 90 group (P> 0.05). The MCAO + 2CCAO * 120 group had 60% mortality in blocking the same artery (MCA + bilateral CCA occlusion); MCAO + 2CCAO * 60 group had less infarction volume and motor function compared with MCAO + 2CCAO * 30 group , The difference was statistically significant (P <0.01). MCAO + 2CCAO * 60 group compared with MCAO + 2CCAO * 90 group, the difference was not statistically significant (P> 0.05). Conclusions MCAO + MCAO + MCAO + MCAO + MCAO + MCAO + MCAO can produce relatively obvious infarct volume and motor function damage with low mortality and short modeling time, which is suitable for ischemic stroke .