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目的探讨胃癌患者外周血中微转移的检测和意义。方法以CD44v6基因作为目的基因,以荧光定量RT-PCR方法检测40例胃癌患者手术前、后外周血及34例同期正常对照者的外周血标本。结果34例对照者外周血中未检出CD44v6mRNA表达,40例胃癌患者术前外周血中都检出有CD44v6mRNA基因的表达,强度在4.9×102~3.2×105拷贝/μg RNA,平均表达量3.9×104拷贝/μg RNA,术后CD44v6mRNA表达强度明显下降,强度在5.5×100拷贝/μg RNA~7.6×103拷贝/μgRNA,平均2.4×102拷贝/μg RNA,术后与术前比较有显著性差异(P<0.01)。结论检测胃癌患者外周血中CD44v6基因的定量表达,可作为胃癌负荷、疗效的参数指标,为临床分期、判断预后提供依据。
Objective To investigate the detection and significance of micrometastasis in peripheral blood of patients with gastric cancer. Methods The CD44v6 gene was used as the target gene to detect the peripheral blood of 40 cases of gastric cancer patients before and after surgery and 34 cases of normal controls at the same period by fluorescence quantitative RT-PCR. Results No CD44v6 mRNA was detected in the peripheral blood of 34 controls. The expression of CD44v6 mRNA was detected in preoperative peripheral blood of 40 patients with gastric cancer, the intensity was 4.9 × 102 ~ 3.2 × 105 copies / μg RNA with the average expression of 3.9 × 104 copies / μg RNA, the intensity of CD44v6 mRNA expression decreased significantly after operation, the intensity was 5.5 × 100 copies / μg RNA to 7.6 × 103 copies / μg RNA, with an average of 2.4 × 102 copies / μg RNA. Difference (P <0.01). Conclusion Quantitative expression of CD44v6 gene in peripheral blood of patients with gastric cancer can be used as a parameter index of gastric cancer burden and curative effect and provide basis for clinical stage and prognosis.