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[目的]探讨大肠癌组织中DPC4蛋白的表达及其与患者临床病理特征的关系。[方法]免疫组化Elivison二步法检测60例大肠癌手术切除标本中DPC4蛋白的表达,并探讨其与患者组织学类型、浸润深度、淋巴结转移和远处转移等临床病理特征的关系。并取40例癌旁正常大肠组织作对照。[结果]DPC4蛋白主要在大肠癌和癌旁正常大肠组织的胞浆表达,呈棕黄色细颗粒。60例大肠癌标本中DPC4的阳性表达为21例(35.0%),其中强阳性为4例(6.7%)。40例癌旁正常大肠组织中DPC4蛋白表达均为阳性,其中强阳性34例(85.0%)。大肠癌组织中DPC4蛋白阳性表达率明显低于癌旁正常大肠组织(χ2=42.62,P<0.01)。大肠癌组织中DPC4蛋白表达水平随组织分化程度的增加而增加(P<0.01),随原发病灶的浸润深度增加、淋巴结转移和远处转移的出现而下降(P<0.01)。[结论]且DPC4蛋白表达降低或缺失在大肠癌的发生发展过程起重要作用,有望成为大肠癌基因诊断和治疗的新靶点。
[Objective] To investigate the expression of DPC4 protein in colorectal carcinoma and its relationship with clinicopathological features. [Method] The immunohistochemical Elivison two-step method was used to detect the expression of DPC4 protein in 60 cases of colorectal cancer resected specimens and to explore its relationship with clinicopathological features such as histological type, depth of invasion, lymph node metastasis and distant metastasis. 40 cases of adjacent normal colorectal tissues were taken as control. [Result] The DPC4 protein was mainly expressed in the cytoplasm of colorectal cancer and adjacent normal colorectal tissues with brownish yellow granules. The positive expression of DPC4 in 60 cases of colorectal cancer was 21 cases (35.0%), of which 4 cases (6.7%) were strongly positive. The expression of DPC4 protein in 40 cases of adjacent normal colorectal tissues was positive, of which 34 cases were strongly positive (85.0%). The positive rate of DPC4 protein in colorectal cancer tissues was significantly lower than that in adjacent normal colorectal tissues (χ2 = 42.62, P <0.01). The expression of DPC4 protein in colorectal carcinoma increased with the degree of differentiation (P <0.01), and decreased with the depth of primary lesion, lymph node metastasis and distant metastasis (P <0.01). [Conclusion] The decrease or deletion of DPC4 protein expression plays an important role in the carcinogenesis and progression of colorectal cancer, which is expected to be a new target for gene diagnosis and treatment of colorectal cancer.