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Phytoene synthase (PSY) is the crucial plastidial enzyme in the carotenoid biosynthetic pathway.However,its post-translational regulation remains elusive.Likewise,Clp protease constitutes a central part of the plastid protease network,but its substrates for degradation are not well known.In this study,we report that PSY is a substrate of the Clp protease.PSY was uncovered to physically interact with various Clp protease subunits (i.e.,ClpS1,ClpC1,and ClpD).High levels of PSY and several other carotenogenic enzyme proteins overaccumulate in the clpc1,clpp4,and clpr1-2 mutants.The overaccumulated PSY was found to be partially enzymatically active.Impairment of Clp activity in clpc1 results in a reduced rate of PSY protein tuover,further supporting the role of Clp protease in degrading PSY protein.On the other hand,the ORANGE (OR) protein,a major post-translational regulator of PSY with holdase chaperone activity,enhances PSY protein stability and increases the enzymatically active proportion of PSY in clpc1,counterbalancing Clp-mediated proteolysis in maintaining PSY protein homeostasis.Collectively,these findings provide novel insights into the quality control of plastid-localized proteins and establish a hitherto unidentified post-translational regulatory mechanism of carotenogenic enzymes in modulating carotenoid biosynthesis in plants.