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利用SSR技术,在抗CMV烟草中发掘与抗病基因紧密连锁的分子标记,目的是为抗病基因的定位克隆以及培育抗病新品种奠定基础。本研究以抗CMV的烤烟品种台烟8号、感病品种NC82为亲本,构建BC1代分离群体,对其进行抗性遗传分析。对分布于烟草24条连锁群上的2317个SSR位点进行了多态性筛选,获得了62个稳定多态性标记,利用这些标记对288株BC1群体分型数据,用WinQTLCart2.5进行分析,找到一个与抗性相关的QTL,LOD值为2.6。研究结论表明,台烟8号对CMV的抗性由多基因控制,标记53735与抗性QTL紧密连锁,遗传距离为0.1 cM,该QTL位于10号连锁群,贡献率为13%。
Using SSR technology, molecular markers closely linked to disease resistance genes were found in anti-CMV tobacco. The purpose is to lay a foundation for the positional cloning of disease-resistant genes and breeding new disease-resistant varieties. In this study, the anti-CMV flue-cured tobacco cultivar Taiyan 8 and the susceptible cultivar NC82 were used as parents to construct the BC1-segregating population and carry out resistance genetic analysis. Polymorphism screening was performed on 2317 SSR loci distributed on 24 linkage groups in tobacco and 62 stable polymorphic markers were obtained. Based on these markers, 288 BC1 population typing data were analyzed by WinQT LCart 2.5 , Find a QTL associated with resistance, LOD value of 2.6. The results showed that the resistance to CMV in Taiyan 8 was controlled by multiple genes. The marker 53735 was closely linked to the resistant QTL with a genetic distance of 0.1 cM. The QTL was located in linkage group 10 with a contribution rate of 13%.