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目的 探讨低能超声对人肺癌细胞的影响。方法 用低能量连续波超声处理小细胞肺癌细胞系SM、腺癌细胞系A2 及人肺纤维母细胞FB ,用胎盘蓝染料排斥试验及克隆存活试验判定效果。结果 低能量 ( 0 .8W /cm2 )超声 3分钟对各种人肺癌细胞及人肺纤维母细胞有杀伤作用 (P <0 .0 5 ) ,10分钟可杀死全部细胞 (P <0 .0 0 1)。细胞浓度越高 ,杀伤作用越低。超声 10分钟后含细胞悬液的试管内温度未见显著上升。声强 0 .8W /cm2 的超声使阿霉素对肺癌细胞SM的杀伤作用增加 10 0倍 (P <0 .0 0 1)。结论 低能连续波超声可杀伤肺癌细胞 ,并能显著增加阿霉素对肺癌细胞的细胞毒性。
Objective To investigate the effect of low-energy ultrasound on human lung cancer cells. Methods Small cell lung cancer cell line SM, adenocarcinoma cell line A2 and human lung fibroblast FB were treated with low-energy continuous wave sonication. The effect was determined by placental blue dye exclusion test and clone survival test. Results Low-energy (0.8W/cm2) ultrasound for 3 minutes had a killing effect on various human lung cancer cells and human pulmonary fibroblasts (P < 0.05), and all cells were killed within 10 minutes (P <0. 0 1). The higher the cell concentration, the lower the killing effect. Ten minutes after sonication, there was no significant increase in the in-tube temperature of the cell suspension. Ultrasound with a sound intensity of 0.8 W/cm2 increased the killing effect of adriamycin on lung cancer cells SM by 100 times (P < 0.01). Conclusion Low-energy continuous wave ultrasound can kill lung cancer cells and significantly increase the cytotoxicity of adriamycin to lung cancer cells.