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2005～2007年对上海崇明水仙病毒病的种类进行了调查和鉴定。崇明水仙病毒病田间主要表现为花叶、斑驳、黄条和白条等多种症状,以花叶和斑驳为主。采用ELISA和RT-PCR方法,从36个水仙样本上检测到水仙普通潜隐病毒(NCLV)(GenBank登录号:EU200454)、水仙退化病毒(NDV)(GenBank登录号:EU200456);此外,有两类分离物被初步鉴定为新病毒,暂命名为水仙斑驳相关病毒(NMaV)(GenBank登录号:EU182651)和水仙白条相关病毒(NWSaV)(GenBank登录号:EU182652)。4类病毒的总检出率分别为11.1%、13.9%、100%和2.8%,其中NMaV为危害水仙的优势病毒。约20%的样本检测出有2种以上病毒的复合侵染,但是未检测出Potexvirus属的病毒。
From 2005 to 2007, the species of dung Chongming daffodil disease in Shanghai were investigated and identified. Chongming narcissus virus disease mainly in the field of mosaic, mottled, yellow and white stripes and other symptoms, with mosaic and mottled. Narcissus common latent virus (NCLV) (GenBank accession number: EU200454) and daffodil degenerating virus (NDV) (GenBank accession No .: EU200456) were detected from 36 samples of Narcissus by ELISA and RT-PCR. In addition, The isolates were tentatively identified as new viruses, and named as NMaV (GenBank accession number: EU182651) andNBSaV (GenBank accession number: EU182652) tentatively named. The total detection rates of 4 kinds of viruses were 11.1%, 13.9%, 100% and 2.8% respectively, among which NMaV was the dominant virus that endangered narcissus. About 20% of the samples detected a combination of two or more viruses, but no Potexvirus was detected.