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设计不同激素、不同水平的单因子试验和正交试验,统计盐桦出愈率、分化率,筛选出诱导盐桦愈伤组织的最适外植体和最适培养基。盐桦愈伤组织高效诱导和不定芽分化的最适外植体为茎段;诱导愈伤组织的最适培养基为:LS+BA 0.5 mg/L+NAA 0.4;愈伤组织分化抽枝的最适培养基为:LS+BA 0.5 mg/L+NAA0.02 mg/L;高效诱导愈伤组织和不定芽分化的最适培养基为:LS+BA 0.5 mg/L+NAA 0.2 mg/L。琼脂7%,蔗糖浓度20 g/L。愈伤组织诱导率和不定芽分化率分别达到82%和93.6%以上。
Different hormones and different levels of single factor test and orthogonal experiment were designed. The rate of emergence and differentiation of birch bark was calculated, and the optimum explants and optimum medium for callus induction were selected. The optimum explants for callus induction and adventitious bud differentiation were the stem segments. The optimum medium for inducing callus was LS + BA 0.5 mg / L + NAA 0.4. The differentiation of callus The optimum culture medium was: LS + BA 0.5 mg / L + NAA 0.02 mg / L; the best medium for inducing differentiation of callus and adventitious buds efficiently was LS + BA 0.5 mg / L + NAA 0.2 mg / L . Agar 7%, sucrose concentration 20 g / L. Callus induction rate and adventitious bud differentiation rate reached 82% and 93.6% respectively.