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用溶胀剂溶胀单分散的聚苯乙烯微球,使稀土配合物铕-二苯甲酰甲烷-邻菲罗啉[Eu(DBM)3phen]渗透进入微球中,制备出含铕配合物的聚苯乙烯荧光微球。系统的研究了制备过程中溶胀剂的选择和用量、Eu(DBM)3phen的用量及溶胀时间对微球形貌和荧光强度的影响。用环境扫描电子显微镜、荧光显微镜和荧光分光光度计对微球的形貌、粒径和荧光强度进行了表征。结果表明,通过条件优化,可以制备出单分散的高亮度荧光微球,微球中铕配合物的最大含量为5.5%,并且荧光微球在pH=1～14范围内均有良好的稳定性,在生物医学领域有很好的应用前景。
The monodisperse polystyrene microspheres were swollen with the swelling agent to infiltrate the rare earth complex Eu (DBM) 3phen into europium-dibenzoylmethane-phenanthroline Styrene fluorescent microspheres. The effects of swelling agent selection and dosage, the amount of Eu (DBM) 3phen and the swelling time on the morphology and fluorescence intensity of the microspheres were investigated systematically. The morphology, particle size and fluorescence intensity of the microspheres were characterized by scanning electron microscopy, fluorescence microscopy and fluorescence spectrophotometer. The results showed that monodisperse high brightness fluorescent microspheres could be prepared by the optimized conditions. The maximum content of europium complexes in the microspheres was 5.5%, and the fluorescent microspheres had good stability in the range of pH = 1-14 , Has a good application prospect in the field of biomedicine.