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凝胶渗透色谱法测定壳聚糖分子量 ,以葡聚糖为标准品 ,用 2种不同的凝胶柱 (PLaquagel- OH柱和 TSK G4 0 0 0 PWXL柱 )测定 4种样品的分子量及其分布 ,并经过普适标定。比较结果发现 :PL aquagel- OH柱效果稍好于 TSK G4 0 0 0 PWXL柱。两柱所得重均分子量平均偏差为 0 .17%～ 3.38% ,数均分子量的平均偏差为 0 .90 %～ 3.5 7% ,分子量分布宽度指数 1.34～ 1.5 4。
Gel permeation chromatography was used to determine the molecular weight of chitosan. Dextran was used as a standard. The molecular weight and distribution of the four samples were determined by two different gel columns (PLaquagel-OH and TSK G4 0 0 0 PWXL) , And after universal calibration. The results showed that PL aquagel-OH column was slightly better than TSK G4 0 0 0 PWXL column. The average weight-average molecular weight deviation of the two columns was from 0.17% to 3.38%, the average deviation of number-average molecular weight was from 0.90% to 3.57%, and the molecular weight distribution width index was from 1.34 to 1.54.