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脂多糖LPS是G~-厌氧菌的重要毒力因子,脂蛋白LPP和脂多糖都是细菌胞膜的结构成分,LPP和LPS是否类似。在牙周病的发生发展中是否起一定作用,本试验分离鉴定齿螺旋体表面的LPP并检测它对人中性白细胞的作用。 材料及方法 选用齿螺旋体标准株ATCC-35404和临床分离株GM-1厌氧培养3～4 d后收集、离心、清洗、非离子清洁剂Trison X-114提取,[~3H]-顺式-9-十八烷酸标记,提取物用丙酮沉淀,己烷异丙醇去除非共价结合的脂质,得到所需的脂蛋白(dLPP)混合物。将dLLP进行SDS-PAGE分离鉴定,采用透析以及疏水
Lipopolysaccharide LPS is an important virulence factor of G ~ - anaerobic bacteria, lipoproteins LPP and lipopolysaccharide are the structural components of the bacterial membrane, LPP and LPS are similar. Whether it plays a role in the occurrence and development of periodontal disease. In this study, we isolated and identified the LPP on the surface of the spirochaete and tested its effect on human neutrophils. MATERIALS AND METHODS Toxoplasma sp. Standard strain ATCC-35404 and clinical isolates GM-1 were cultured anaerobically for 3 to 4 days and then collected, centrifuged, washed and extracted with non-ionic detergent Trison X-114. The [~ 3H] Octadecanoic acid, the extract was precipitated with acetone and hexanes isopropanol to remove non-covalently bound lipids, resulting in the desired mixture of lipoproteins (dLPPs). The dLLP was separated and identified by SDS-PAGE, using dialysis and hydrophobic