论文部分内容阅读
以果梅品种小叶猪肝为试材 ,研究了果梅SSR技术中PCR反应体系的主要成分对SSR扩增结果的影响 ,并比较了采用聚丙稀酰胺凝胶及琼脂糖电泳检测扩增产物多态性的差异。结果表明 :在PCR反应体系中 ,Mg2 + 的最适浓度范围为1 89～ 2 83mmol·L-1;dNTP最适浓度为 0 2 4mmol·L-1;引物的最适浓度为 0 38μmol·L-1;Taq 聚合酶在 2 6 5 μL反应体系中宜加入 1U。利用此反应体系 ,对 2 4个果梅代表品种进行了SSR反应 ,用 8%的非变性聚丙稀酰胺凝胶电泳检测 ,扩增产物在 10 0～ 2 0 0bp之间 ,不同品种间DNA谱带多态性丰富。琼脂糖电泳检测的DNA多态性不如聚丙稀酰胺凝胶丰富
The effects of the main components of the PCR reaction system on the SSR amplification results of the plum plum piglets were studied using the plumule liver of the plum varieties. The polymorphism of the amplified product was compared by using polyacrylamide gel and agarose gel electrophoresis The difference. The results showed that the optimal concentration of Mg2 + in the PCR reaction ranged from 1 89 to 2 83 mmol·L-1, the optimal concentration of dNTP was 0 2 4 mmol·L-1, and the optimum concentration of primer was 0 38 μmol·L -1; Taq polymerase in 2 6 5 μL reaction system should be added 1U. SSR reactions were performed on 24 representative cultivars of Prunus mume using this reaction system. The PCR products were detected by 8% non-denaturing polyacrylamide gel electrophoresis. The amplified products ranged from 10 0 to 200 bp. The DNA bands of different cultivars Rich polymorphism. DNA polymorphisms detected by agarose gel electrophoresis are not as abundant as polyacrylamide gels