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目的:探讨宫颈鳞癌组织中p16INK4A,NF-κB p65的表达及临床病理学意义。方法:采用免疫组化PV-9000法检测p16INK4A,NF-κB p65在30例宫颈鳞状细胞癌(SCC)、30例宫颈上皮内瘤病变(CIN)和30例慢性宫颈炎(对照组)中的表达。结果:在慢性宫颈炎组织、CIN和宫颈鳞癌中p16INK4A的阳性率分别为26.66%、50.00%及93.33%,其表达差异有统计学意义(P<0.01)。NF-κB p65的阳性率分别为23.33%、56.66%及86.66%,其表达差异有统计学意义(P<0.01)。p16INK4A、NF-κB p65在不同临床分期,病理分级及淋巴结是否转移中的阳性表达率差异均无统计学意义(P>0.05)。p16INK4A表达与是否绝经有关,在绝经组中表达高于未绝经组(P<0.01)。结论:p16INK4A、NF-κB p65的表达在宫颈癌的发生、发展中起重要作用,p16INK4A和NF-κBp65有可能成为预测宫颈鳞癌发生的生物学检测指标。
Objective: To investigate the expression of p16INK4A and NF-κB p65 in cervical squamous cell carcinoma and its clinicopathological significance. Methods: The expression of p16INK4A and NF-κB p65 in 30 cases of cervical squamous cell carcinoma (SCC), 30 cases of cervical intraepithelial neoplasia (CIN) and 30 cases of chronic cervicitis (control group) were detected by immunohistochemical PV-9000 method expression. Results: The positive rates of p16INK4A in chronic cervicitis, CIN and cervical squamous cell carcinoma were 26.66%, 50.00% and 93.33%, respectively. The positive rates of p16INK4A were statistically significant (P <0.01). The positive rate of NF-κB p65 was 23.33%, 56.66% and 86.66%, respectively. The difference was statistically significant (P <0.01). The positive rates of p16INK4A and NF-κB p65 in different clinical stages, pathological grade and lymph node metastasis showed no significant difference (P> 0.05). p16INK4A expression is related to whether menopause, in menopause group is higher than that in menopause group (P <0.01). Conclusion: The expression of p16INK4A and NF-κB p65 plays an important role in the occurrence and development of cervical cancer. P16INK4A and NF-κBp65 may be the biological markers for predicting the occurrence of cervical squamous cell carcinoma.