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[目的]为提高芒草的酶解产糖得率,研究不同预处理对其影响。[方法]采用射线辐照与碱性双氧水对芒草进行协同预处理,考察对其酶解产糖的影响。[结果]经400 k Gy剂量射线辐照预处理后,芒草酶解产还原糖的得率为76.24 mg/g;采用400 k Gy剂量射线辐照与碱性双氧水协同预处理,芒草酶解产还原糖的得率为505.08 mg/g,较只进行相同剂量射线辐照处理提高了5.6倍。通过工艺优化,得出较优水解条件为:预处理温度30℃、Na OH浓度1.2%、双氧水浓度2%、处理时间6 h。[结论]射线辐照与碱性双氧水协同预处理能够大大提高芒草酶解产糖效率,为利用其制备燃料乙醇提供了新的理论依据。
[Objective] The research aimed to study the effects of different pretreatments on the yield of Miscanthus sinensis enzymolysis. [Method] Radiation and alkaline hydrogen peroxide were used to pre-treat Miscanthus to investigate its effect on the yield of sugar. [Result] The yield of reducing sugars of Miscanthus mannii was 76.24 mg / g pretreated with 400 k Gy dose radiation. The synergistic pretreatment with 400 k Gy dose radiation and alkaline hydrogen peroxide, The yield of reducing sugar was 505.08 mg / g, which was 5.6 times higher than that of irradiation with the same dose. Through the process optimization, the optimal hydrolysis conditions were: pretreatment temperature 30 ℃, NaOH concentration 1.2%, H2O2 concentration 2%, treatment time 6 h. [Conclusion] The synergistic pretreatment with radiation and alkaline hydrogen peroxide could greatly enhance the efficiency of enzymatic hydrolysis of Miscanthus sinensis, which provided a new theoretical basis for the preparation of fuel ethanol.