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1.制备Vi凝集诊断液宜用Vi—Ⅰ株菌,用福尔属林和氯化钙处理保存。 2.被检血清预先加热56℃,30分钟为宜,再以H、O抗原吸收后进行Vi凝集反应较更为可靠;作为有诊断意义的滴度宜根据所用的诊断液、操作方法等具体情况而定。 3.血清Vi凝集反应可作为发现伤寒带菌者可靠的辅助方法,可用于大量细菌学检查前选择对象的方法;可作为伤寒患者病愈出院和管理带菌者的指标之一;可作为伤寒患者病程中免疫状态的参考。 4.血清Vi凝集反应阳性时,很可能为带菌者,但不能肯定其一定是带菌者,而尚需用其他方法进一步证实,Vi凝集反应阴性也不能绝对排除是带菌者。
1. Preparation of Vi agglutination diagnostic solution should Vi-Ⅰ strain, with Folium Lin and calcium chloride treatment preservation. 2. Pre-heated serum was seized at 56 ℃, 30 minutes is appropriate, and then H, O antigen absorption after the Vi agglutination reaction is more reliable; as a diagnostic titer should be based on the diagnostic fluid used, methods of operation and other specific Depending on the situation. Serum Vi agglutination can be used as a reliable auxiliary method for detecting typhoid carriers, which can be used to select objects before mass bacteriological examination. It can be used as an indicator of cured patients discharged from hospital and management of carriers. In the immune status of the reference. Serum Vi agglutination reaction is positive, it is likely to be carriers, but can not be sure they must be carriers, but still need to use other methods to further confirm that Vi agglutination negative can not be absolutely excluded carriers.