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目的研究西洛司特在体外对COPD患者PBMC分泌IL-8和TNF-α的影响。方法从12例COPD患者外周血中分离的单个核细胞(PBMC)分别与不同浓度的西洛司特、氨茶碱和甲基强的松龙共培养24 h,用ELISA的方法检测PBMC培养上清的IL-8和TNF-α水平。结果西洛司特和氨茶碱对PBMC分泌IL-8和TNF-α均呈显著的浓度依赖性抑制作用(P<0.01)。甲基强的松龙对PBMC分泌IL-8和TNF-α则无显著抑制作用。1μmol/L的西洛司特使LPS刺激PBMC活化分泌的IL-8减少54%,15μg/m l的氨茶碱可使其减少39%,两者在治疗剂量所能达到的血药浓度比较有显著性差异(P<0.01)。1μmol/L的西洛司特使LPS刺激PBMC活化分泌的TNF-α减少51%,15μg/m l的氨茶碱可使其减少32%,两者在治疗剂量所能达到的血药浓度比较有显著性差异(P<0.01)。结论西洛司特和氨茶碱在体外可显著地抑制抑制PBMC分泌IL-8和TNF-α,且西洛司特的抑制作用显著强于氨茶碱,甲基强的松龙这种抗炎作用不明显,提示西洛司特对COPD患者有显著的抗炎活性,具有广阔的临床应用前景。
Objective To investigate the effects of cilazapril on PBMC IL-8 and TNF-α secretion in COPD patients in vitro. Methods Peripheral blood mononuclear cells (PBMCs) isolated from 12 patients with chronic obstructive pulmonary disease (COPD) were co-cultured with cilostazol, aminophylline and methylprednisolone at different concentrations for 24 h. ELISA was used to detect PBMC culture Clear IL-8 and TNF-α levels. Results Cilostazol and aminophylline significantly inhibited IL-8 and TNF-α production in PBMCs in a concentration-dependent manner (P <0.01). Methylprednisolone did not significantly inhibit the secretion of IL-8 and TNF-α by PBMCs. 1μmol / L of cilostazol reduced LPS-stimulated PBMC activation and secretion of IL-8 by 54%, 15μg / ml of aminophylline can reduce 39%, both at the therapeutic dose can reach the plasma concentration was more significant Sex differences (P <0.01). 1μmol / L of cilostazol reduced LPS-stimulated PBMC activation and secretion of TNF-α by 51%, 15μg / ml of aminophylline can make it reduce by 32%, both at the therapeutic dose can reach the plasma concentration was more significant Sex differences (P <0.01). Conclusion Cilostazol and aminophylline can significantly inhibit the secretion of IL-8 and TNF-α in PBMC in vitro, and the inhibitory effect of cilostazol is stronger than that of aminophylline and methylprednisolone Inflammatory effect is not obvious, suggesting that cilostazol in patients with COPD significant anti-inflammatory activity, has broad prospects for clinical application.