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目的探讨转染高表达neuritin基因的雪旺细胞在高糖情况下对背根神经细胞的影响。方法构建neuritin基因的高表达系统,包装出含有目的基因的慢病毒载体,转染雪旺细胞。流式细胞计数法(FACS)检测雪旺细胞的转染率。分离纯化SD大鼠的背根神经细胞,与转染高表达neuritin基因的雪旺细胞或正常雪旺细胞共培养。高糖情况下分别观察其对背根神经细胞生长的影响。细胞免疫荧光化学分析背根神经元突起的生长情况。ELISA检测细胞培养上清液中neuritin的浓度。结果高糖情况下,与非转染雪旺细胞/背根神经细胞共培养相比,转染高表达neuritin基因雪旺细胞/背根神经细胞共培养的培养液中neuritin的含量增高,并且促进背根神经细胞突起的生长(P<0.05)。结论在高糖情况下,转染neuritin基因的雪旺细胞能高分泌neuritin蛋白,并促进背根神经节的生长。
Objective To investigate the effect of transfection of Schwann cells with high expression of neuritin gene on the dorsal root cells under high glucose conditions. Methods The high expression system of neuritin gene was constructed and the lentiviral vector containing the target gene was packaged and transfected into Schwann cells. Flow cytometry (FACS) was used to detect the transfection rate of Schwann cells. Dorsal root neurons isolated and purified from SD rats were co-cultured with Schwann cells or normal Schwann cells transfected with high-expression neuritin gene. The effects of high glucose on the growth of dorsal root neurons were observed. Immunofluorescence Chemiluminescence Analysis of Dorsal Root Protrusion Protrusion. The concentration of neuritin in the cell culture supernatant was measured by ELISA. Results Compared with non-transfected Schwann cells / dorsal root nerve cells, the levels of neuritin in transfected cells with high expression of neuritin gene Schwann cells / dorsal root neurons were increased Dorsal root ganglion neurite growth (P <0.05). Conclusion In high glucose conditions, Schwann cells transfected with neuritin gene can secrete neuritin protein and promote the growth of DRG.