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以牛血清白蛋白(BSA)为包裹剂,液相还原硝酸银制备了椭圆形的银-牛血清白蛋白(BSA-Ag NPs)纳米粒子。探究了BSA的浓度、溶液pH值和温度对纳米Ag粒子晶体生长的影响,用透射电镜(TEM)、傅里叶红外光谱仪(ATR-FTIR)和热重分析仪(TG)表征了纳米粒子的结构和形貌,用荧光淬灭法分析了纳米粒子的构象和作用机理,建立了位点结合模型,证明纳米粒子发生的是静态荧光淬灭。通过测量大肠杆菌培养基吸光度,发现BSA-Ag NPs纳米粒子对大肠杆菌具有很强的抑制作用,其抗菌性随粒径减小逐渐增强。结果表明:合成的BSA-Ag NPs纳米粒子粒径大约5nm,纳米粒子形态稳定,分散性好,抗菌性强,是一种用途广泛、环境友好型和能直接投入应用的抗菌性材料。
Oval silver-bovine serum albumin (BSA-Ag NPs) nanoparticles were prepared by liquid-phase reduction of silver nitrate with bovine serum albumin (BSA) as coating agent. The effects of BSA concentration, solution pH and temperature on the crystal growth of nano-Ag particles were investigated. TEM, Fourier transform infrared spectroscopy (ATR-FTIR) and thermal gravimetric analysis (TG) Structure and morphology, the conformation and mechanism of the nanoparticles were analyzed by fluorescence quenching method, and the site binding model was established to prove that the static fluorescence quenching occurred in the nanoparticles. By measuring the absorbance of E. coli medium, it was found that BSA-Ag NPs nanoparticles have a strong inhibitory effect on E. coli, and its antibacterial activity gradually increases with decreasing particle size. The results showed that the synthesized nanoparticles of BSA-Ag NPs were about 5nm in size with stable morphology, good dispersibility and strong antibacterial activity. The nanoparticles were widely used, environment-friendly and directly applied.