论文部分内容阅读
以大豆卵磷脂和胆固醇为膜材,采用薄膜蒸发-动态高压法制备VC纳米脂质体。通过单因素考察处方工艺对包封率的影响以及正交设计法进行处方工艺优化。结果表明,制备VC纳米脂质体的最优条件:制备温度60℃,VC浓度5mg/mL,VC和总脂材质量比为1∶10,大豆卵磷脂和胆固醇质量比为4∶1,表面活性剂和总脂材质量比为4∶10,动态高压处理压力为140MPa,处理2次。该条件下包封率可达(47.16±6.28)%,平均粒度为(73.9±4.4)nm。
VC and liposomes were prepared by thin-film evaporation-dynamic high-pressure method using soybean lecithin and cholesterol as membrane materials. Through the single-factor study of the impact of prescription process on the entrapment efficiency and orthogonal design method for prescription optimization process. The results showed that the optimum conditions for the preparation of VC nanosomes were as follows: preparation temperature 60 ℃, VC concentration 5mg / mL, mass ratio of VC to total lipid 1:10, mass ratio of soybean lecithin to cholesterol 4:1, surface Active agent and total fat mass ratio of 4:10, dynamic high-pressure processing pressure of 140MPa, treatment twice. Under these conditions, the encapsulation efficiency was (47.16 ± 6.28)% and the average particle size was (73.9 ± 4.4) nm.