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目的:本文从补肾壮阳胶囊(WSKY)改善精神分裂症模型小鼠认知功能障碍进行研究,以探究其可能的作用机制。方法:采用地卓西平马来酸盐(MK-801)建立精神分裂症模型。动物行为学方面,运用Morris水迷宫实验评价补肾壮阳胶囊对精神分裂症小鼠认知功能障碍的治疗效应;组织生物化学方面,运用实时荧光定量PCR(RT-PCR)和蛋白免疫印迹(Western blot)的方法考察补肾壮阳胶囊对脑源性神经营养因子(BDNF)、钙调蛋白激酶Ⅱ(CaMKⅡ)、环腺苷酸反应元件结合蛋白(CREB)的mRNA和细胞外信号调节激酶(ERK)蛋白表达的影响。结果:WSKY中、高剂量组小鼠Morris水迷宫定位航行逃避潜伏期小于模型组,而其空间探索穿过原平台所在位置的次数、在原平台所在象限搜索的时间百分比均大于模型组(P<0.05);WSKY中、高剂量可以增加BDNF mRNA的含量和ERK蛋白的表达。结论:WSKY中、高剂量可以改善精神分裂症小鼠的认知功能障碍,其作用机制与WSKY提高精神分裂症小鼠海马区BDNF mRNA的表达和增加ERK蛋白含量有关。
OBJECTIVE: To study the cognitive dysfunction of Bushen ZHENGYANG capsule (WSKY) in schizophrenia model mice to explore its possible mechanism. Methods: Dextromethorphan maleate (MK-801) was used to establish schizophrenia model. In animal behavior, Morris water maze test was used to evaluate the therapeutic effect of Bushen Zhuangyang Capsule on cognitive dysfunction in schizophrenic mice. In the aspect of tissue biochemistry, real-time quantitative PCR (RT-PCR) and Western blot ) On the expression of BDNF, CaMKⅡ, CREB mRNA and ERK protein The impact of expression. Results: Morris water maze localization of WSKY mice in the high-dose and high-dose groups was less than that in the model group, and the number of space exploration through the original platform was longer than that in the model group (P <0.05) ); WSKY, high dose can increase BDNF mRNA content and ERK protein expression. Conclusion: WSKY at high dose can improve cognition dysfunction in schizophrenic mice, and its mechanism may be related to the increase of BDKF expression and the increase of BDNF mRNA level in hippocampus of mice with schizophrenia by WSKY.