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本实验采用分离细胞体外培养和MTT微量比色法,观察了新生大鼠视网膜(R)、顶盖浅灰质(T)、间脑后外侧部(G)和枕叶皮质(C)等细胞体外培养生长的相互影响。培养3天后,MTT微量比色法测定发现:分别取自视觉系统两个部分的混合细胞培养,除G+C和T+G生长不活跃外,其余的混合培养均比单独培养的细胞生长明显活跃(高1.3～3倍不等)。其中R+T和R+G生长最为活跃,其次为R+C和T+C。另外T的条件培养液(Tc)可使R细胞生长活跃3倍,使G细胞生长活跃2倍。R的条件培养液(Rc)增强G和C细胞生长活性分别为1.3倍和2倍。以上的实验表明,T和R的条件培养液中,可能有神经诱向因子存在。
In this study, cultured cells were cultured in vitro and MTT micro-colorimetric method was used to observe the effects of retinal (R), capital gray matter (T), lateral posterolateral part (G) and occipital cortex (C) Cultivation of the interaction of growth. After three days of culture, MTT microcolorimetric assay showed that mixed cultures from two parts of the visual system, except G + C and T + G, did not grow well, and the rest of the mixed cultures grew more than the cultured cells alone Active (high 1.3 to 3 times). Among them, R + T and R + G were most active, followed by R + C and T + C. In addition T conditioned medium (Tc) can make R cell growth 3 times active, so that G cell growth 2 times. R conditioned medium (Rc) enhanced the growth activity of G and C cells by 1.3 and 2 times, respectively. The above experiments show that, T and R conditioned medium, there may be neurotrophic factor exists.