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目的:建立测定人血浆中舒芬太尼浓度的高效液相色谱法。方法:血浆样品经正己烷-乙醇(95:5,)提取后,以乙腈-5 mmol·L~(-1)磷酸二氢钾缓冲液(40:60,pH 6.1)为流动相,流速1.0 ml·min~(-1),色谱柱为Agilent Extend C_(18)(150 mm×4.6 mm,5μm),柱温25℃,检测波长230 nm。结果:血浆内源性杂质不干扰待测物测定,舒芬太尼的线性范围为2.014～201.400 g·L~(-1),定量下限为2.014 g·L~(-1),日内、日间RSD均小于6%。样品3次冻融及在提取后,4℃下12 h内稳定性良好。结论:该法灵敏、快速、准确,操作简便,可用于舒芬太尼的血药浓度监测和临床药动学研究。
Objective: To establish a HPLC method for the determination of sufentanil in human plasma. Methods: After the plasma samples were extracted with n-hexane-ethanol (95: 5,), acetonitrile-5 mmol·L -1 potassium dihydrogen phosphate buffer (40:60, pH 6.1) ml · min ~ (-1) .The column was Agilent Extend C_ (18) (150 mm × 4.6 mm, 5μm). The column temperature was 25 ℃ and the detection wavelength was 230 nm. Results: Plasma endogenous impurities did not interfere with the determination of analytes. The linear range of sufentanil was 2.014 ~ 201.400 g · L -1, and the lower limit of quantitation was 2.014 g · L -1. Between RSDs are less than 6%. The samples were freeze-thawed three times and the stability was good within 12 h at 4 ° C after extraction. Conclusion: The method is sensitive, rapid, accurate and easy to operate. It can be used to monitor the concentration of sufentanil and clinical pharmacokinetics.