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Purpose: Dexamethasone(DEX) was tested for its ability to modulate human retinalpigment epithelium (hRPE)cell proliferation in cell culture.Methods: DEX in different concentrations was added to cultured hRPE cells. The effectswere measured with MTT method, 3H-thymidine(3H-TdR) incorporation and flowcytometry.Results :DEX increased survival rate and DNA synthesis from 32 mg/L to 320 mg/Lunder hRPE culture conditions, but paradoxically reduced them at 1 000 mg/L and3 200 mg/L in dose and time dependent fashion by both MTT assay and 3 H-TdRincorporation. The cell numbers in S phase and G2/M phase increased 28. 32 % at DEXconcentration 320 mg/L, in contrast, reduced 41. 84 % at 1 000 mg/L.Conclusion: DEX increased proliferation from 32 mg/L to 320 mg/L, and inhibitedproliferation at concentrations greater than 320 mg/L. The inhibiting effect of DEX mayhappen in s phase and G2/M phase.
Methods: DEx in different concentrations was added to cultured hRPE cells. The effectswere measured with MTT method, 3H-thymidine (3H -TdR) incorporation and flowcytometry. Results: DEX increased survival rate and DNA synthesis from 32 mg / L to 320 mg / L hRPE culture conditions, but paradoxically reduced them at 1000 mg / L and 300 mg / L in dose and time dependent fashion by both MTT assay and 3 H-TdRincorporation. The cell numbers in S phase and G2 / M phase increased 28. 32% at DEXconcentration 320 mg / L, in contrast, reduced 41. 84% at 1 000 mg / L. Conlusion : DEX increased proliferation from 32 mg / L to 320 mg / L, and inhibitedproliferation at concentrations greater than 320 mg / L. The inhibiting effect of DEX mayhappen in s phase and G2 / M phase.