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目的:观察益智康合剂对实验性智力低下大鼠学习记忆功能的影响,并探讨其益智的作用机理。方法:40天幼龄SD大鼠150只,随机选出12只作为假手术组,余下造模。随机分为模型组、阳性药物组、中药小剂量组、中药中剂量组、中药大剂量组、假手术组。通过Morris水迷宫测试观察了益智康合剂对幼龄智力低下大鼠学习记忆能力的改善作用,经大鼠大脑皮质乙酰胆碱酯酶(AchE)活性、海马中一氧化氮(NO)含量的测定来探讨其改善智能的中枢机制。结果:与假手术组比较,模型组大鼠学习记忆能力降低(P<0.01);其大脑皮质AchE活性升高(P<0.01);海马中NO含量降低(P<0.01)。与模型组比较,益智康合剂能改善幼龄智力低下大鼠的学习记忆能力(P<0.01,P<0.05);降低幼龄智力低下大鼠大脑皮质AchE的活性(P<0.01,P<0.05);提高其海马中NO含量(P<0.01,P<0.05)。结论:益智康合剂能改善幼龄智力低下大鼠的学习记忆能力,其机制可能与降低幼龄智力低下大鼠大脑皮质AchE的活性、提高其海马中NO含量有关。
Objective: To observe the effects of Yi Zhi Kang Mixture on learning and memory in experimental mental retardation rats, and to explore the mechanism of its puzzle. Methods: 150 40-day-old SD rats were randomly selected 12 as the sham-operated group, the rest of the model. Randomly divided into model group, positive drug group, small dose of traditional Chinese medicine group, medium dose group, large dose of traditional Chinese medicine group, sham operation group. Morris water maze test was used to observe the effect of Yizhikang on the learning and memory abilities of young mentally retarded rats. The activity of acetylcholinesterase (AchE) in brain cortex and the content of nitric oxide (NO) in the hippocampus were determined Explore its central mechanism for improving intelligence. Results: Compared with the sham operation group, the learning and memory abilities of the model group decreased (P <0.01), the AchE activity of the cerebral cortex increased (P <0.01), and the content of NO in the hippocampus decreased (P <0.01). Compared with the model group, Yizhikang mixture can improve the learning and memory abilities in young rats with mental retardation (P <0.01, P <0.05), reduce the activity of AchE in cerebral cortex of young rats with mental retardation (P <0.01, P < 0.05), and increased the content of NO in hippocampus (P <0.01, P <0.05). Conclusion: Yizhikang Mixture can improve the learning and memory abilities in young rats with mental retardation. The mechanism may be related to reducing the activity of AchE in the cerebral cortex, and increasing the content of NO in the hippocampus.