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目的:探讨诱导型一氧化氮合酶抑制剂(i NOS)抑制剂对胆管癌细胞生物学行为的影响。方法:不同浓度i NOS抑制剂1400W孵育人胆管癌QBC939细胞24 h后,分别用硝酸还原酶法、MTT比色法检各组细胞中NO浓度与增殖情况,并计算半抑制浓度(IC_(50));根据IC_(50)值,选择合适浓度的1400W处理QBC939细胞24 h后,分别用划痕试验、Transwell小室法检测细胞迁移及侵袭情况。以上实验均以未加1400W培养基处理的QBC939细胞为空白对照。结果:与空白对照组比较,各1400W处理组QBC939细胞中NO含量及增殖率均呈浓度依赖性降低(均P<0.05),IC_(50)值为51.24μmol/L;用_(50)μmol/L的1400 W处理QBC939细胞24 h后,实验组的细胞划痕愈合率(61.7%vs.92.3%)和细胞侵袭数(72.7个vs.128.0个)均较对照组明显降低(均P<0.05)。结论:i NOS抑制剂1400W能抑制胆管癌细胞增殖、迁移和侵袭,其机制可能与NO下游的信号分子变化有关。
Objective: To investigate the effect of inducible nitric oxide synthase inhibitor (iNOS) inhibitor on the biological behavior of cholangiocarcinoma cells. Methods: After incubation of human cholangiocarcinoma QBC939 cells with different concentrations of NOS inhibitor 1400W for 24 h, the concentration and proliferation of NO in each group were measured by nitrate reductase and MTT assay, and the half inhibitory concentration (IC 50 )). According to the value of IC 50, the cells were treated with 1400 W at a proper concentration for 24 h, and then scratch assay and Transwell chamber assay were used to detect the migration and invasion of QBC939 cells. In the above experiment, QBC939 cells treated with no 1400W medium were blank control. RESULTS: Compared with the blank control group, NO content and proliferation rate of QBC939 cells in each treatment group were decreased in a concentration-dependent manner (all P <0.05), IC 50 value was 51.24μmol / L, The cell healing rate (61.7% vs 92.3%) and cell invasion (72.7% vs. 128.0) in QBC939 cells treated with 1400 W / L for 24 h were significantly lower than those in control group (all P < 0.05). CONCLUSION: The iNOS inhibitor 1400W can inhibit the proliferation, migration and invasion of cholangiocarcinoma cells. The mechanism may be related to the changes of signal molecules downstream of NO.