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目的探讨骨髓间充质干细胞对脂多糖(LPS)刺激巨噬细胞分泌炎症因子IL-10、TNF-α的影响。方法体外分离、培养、鉴定骨髓间充质干细胞与巨噬细胞,分PBS、BMSCs、Macrophage、Macrophage+BMSC、BMSCs+LPS、Macrophage+LPS、Macrophage+BMSCs+LPS 7组进行细胞共培养实验。收集不同时间点培养基上清液,ELISA检测其炎症因子IL-10、TNF-α的含量。结果 B(BMSCs)组与E(BMSCs+LPS)组相比,IL-10与TNF-α的含量没有明显变化;F(Macrophage+LPS)组IL-10、TNF-α的含量均高于C组(Macrophage);G(Macrophage+BMSCs+LPS)组与F(Macrophage+LPS)组相比,TNF-α明显降低、IL-10明显升高。结论 BMSCs可以减轻LPS对巨噬细胞分泌炎症因子的刺激作用,减轻炎症反应的程度。
Objective To investigate the effects of bone marrow mesenchymal stem cells on the secretion of inflammatory cytokines IL-10 and TNF-α by macrophages stimulated by lipopolysaccharide (LPS). Methods Bone marrow mesenchymal stem cells and macrophages were isolated and cultured in vitro. The cells were cultured in PBS, BMSCs, Macrophage, Macrophage + BMSC, BMSCs + LPS, Macrophage + LPS, Macrophage + BMSCs + LPS 7 groups. Supernatants of medium at different time points were collected, and the levels of IL-10 and TNF-α in inflammatory cells were detected by ELISA. Results Compared with E (BMSCs + LPS) group, the levels of IL-10 and TNF-α in B (BMSCs) group did not change significantly. The levels of IL-10 and TNF- Macrophage + BMSCs + LPS group was significantly lower than that of F (Macrophage + LPS) group, IL-10 was significantly increased. Conclusion BMSCs can reduce the stimulating effect of LPS on inflammatory cytokines secreted by macrophages and reduce the degree of inflammatory reaction.