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目的:观察褪黑素对体外缺氧诱导的小鼠神经干细胞(neural stem cells,NSCs)增殖和分化的影响。方法:取孕12.5 d胚胎小鼠大脑皮质分离培养NSCs,建立缺氧模型。利用免疫荧光染色,检测分析褪黑素对缺氧诱导后不同时间点的NSCs增殖和分化的影响。结果:缺氧后NSCs的增殖能力明显下降,而褪黑素可显著改善这一现象,促进缺氧后NSCs的增殖。缺氧后NSCs向神经元的分化明显受阻,褪黑素处理组的神经元的分化率明显高于对照组,其中作用高峰期是分化的第七天。结论:体外缺氧的NSCs增殖和分化均明显受到抑制,而褪黑素的干预可明显改善干细胞的增殖,促进NSCs向神经元的分化,但对星形胶质细胞的分化没有显著影响。
Objective: To observe the effect of melatonin on the proliferation and differentiation of neural stem cells (NSCs) induced by hypoxia in vitro. Methods: NSCs were isolated and cultured in the cerebral cortex of embryonic mice 12.5 days old to establish hypoxia model. Immunofluorescence staining was used to detect the effects of melatonin on the proliferation and differentiation of NSCs at different time points after hypoxia induction. Results: The proliferation of NSCs decreased significantly after hypoxia, while melatonin significantly improved this phenomenon and promoted the proliferation of NSCs after hypoxia. After hypoxia, the differentiation of NSCs to neurons was obviously blocked. The differentiation rate of neurons in melatonin treatment group was significantly higher than that in control group, and the peak of action was the seventh day of differentiation. CONCLUSION: The proliferation and differentiation of NSCs in vitro are significantly inhibited. However, the intervention of melatonin can significantly improve the proliferation of NSCs and promote the differentiation of NSCs to neurons, but not significantly affect the differentiation of astrocytes.