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目的:研究C-MET在大鼠胰腺发育阶段的表达和细胞定位。方法:运用RT-PCR和Western Blot技术分别检测C-MET在大鼠胰腺发育阶段的mRNA和蛋白表达水平;运用免疫组化和免疫荧光技术检测不同时期C-MET在胰腺的组织细胞学定位。结果:RT-PCR结果显示E18.5C-MET mRNA高表达。Western Blot结果显示其蛋白在P14,P21高表达,并存在两种亚型,分子量分别为190KD和170KD。免疫组化和免疫荧光结果显示在不同发育时期C-MET在胰岛β细胞和间充质细胞都有表达。结论:C-MET在大鼠胚胎发育后期及生后出现高表达,并表达于胰岛β细胞和间充质细胞,可能参与了胰岛形成、结构重塑和功能维持。
Objective: To study the expression of C-MET in rat pancreatic development and cell location. Methods: The mRNA and protein expression of C-MET in rat pancreas was detected by RT-PCR and Western Blot. The histopathology of C-MET in pancreas was detected by immunohistochemistry and immunofluorescence. Results: RT-PCR results showed that E18.5C-MET mRNA was highly expressed. Western Blot results showed that the protein was highly expressed in P14 and P21 and existed in two subtypes with molecular weights of 190KD and 170KD respectively. Immunohistochemistry and immunofluorescence results showed that C-MET was expressed in both pancreatic β-cells and mesenchymal cells at different developmental stages. CONCLUSION: C-MET is overexpressed in late embryogenesis and postnatal development of rat and expressed in islet beta cells and mesenchymal cells, which may be involved in islet formation, remodeling and function maintenance.