用CO_2激光照豚鼠背部皮肤,右侧为照射区,左侧非照区;在照射前三周取左,右侧皮片,照射20次及40次后分别切取左,右侧皮片。固定于Bouin液,常规制片,分别作H.E及Weigert弹性纤维染色。电镜材料用0.25%戊二醛固定,锇酸后固定,Epon812包埋,超薄切片,醋酸铀 CO 2 laser was applied to the skin of the back of guinea pig. The right side was the irradiated area and the left non-irradiated area. Left and right skin slices were taken three weeks before irradiation, and left and right skin slices were obtained after 20 and 40 shots respectively. Fixed to Bouin solution, routinely prepared for H.E and Weigert elastic fiber staining. Electron microscopy materials were fixed with 0.25% glutaraldehyde, osmium acid fixed, Epon812 embedded, ultra-thin sections, uranyl acetate