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许多研究表明,水母能够耐受低氧环境,但是并不清楚其生理与分子机制.本研究根据海月水母转录组测序结果,获得了海月水母低氧诱导因子(HIF)的部分基因序列,利用实时荧光定量PCR方法检测海月水母HIF基因在低氧条件下(溶解氧约为0.5 mg L-1)的表达变化情况.结果表明,在低氧条件下,海月水母幼体HIF-1α的基因相对表达量经过一定时间的培养后显著高于对照组;以管蛋白基因为内参基因,HIF-1α基因相对表达量在培养3,6,12,18,48和72 h时分别为参照组(饱和溶解氧)的83.2%,102.4%,122.8%,301.3%,140.3%和150.7%.表明低氧环境中海月水母HIF基因表达量在转录水平有极为显著的增加,这为从分子水平揭示水母耐受低氧环境的机制提供了新的视点.
Many studies have shown that jellyfish can tolerate the hypoxic environment, but its physiological and molecular mechanisms are not clear.In this study, based on the sequencing results of the sea jellyfish transcriptome, we obtained some gene sequences of hypoxia-inducible factor (HIF) The changes of HIF-1α expression in hypoxia (dissolved oxygen was about 0.5 mg L-1) were detected by real-time fluorescence quantitative PCR.The results showed that under hypoxic conditions, the expression of HIF-1α The relative expression level of HIF-1α gene was significantly higher than that of the control group after a certain period of incubation. The relative expression level of HIF-1α gene with tube protein gene as reference gene was 3, 6, 12, 18, 48 and 72 h Indicating that there was a very significant increase in the level of HIF gene expression at the transcriptional level in the hypoxia environment as revealed by the molecular level (83.2%, 102.4%, 122.8%, 301.3%, 140.3% and 150.7% The mechanisms by which jellyfish tolerate the hypoxic environment provide a new perspective.