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采用超声波辅助提取技术,考察了料液比、80℃热水浸泡时间、超声时间、超声功率四因素对短毛柽柳花、嫩枝叶、茎中多糖提取率的影响。本实验条件下,短毛柽柳不同部位多糖提取的最佳工艺条件为:料液比1:60,热水浸泡时间1h,超声时间20min,超声功率300W。此时花、嫩枝叶、茎中多糖含量分别为53.06、164.90、48.01mg/g,嫩枝叶的多糖含量明显高于其他两个部位。此方法回收率为98.25%。
Ultrasonic-assisted extraction was used to investigate the effect of four factors on the extraction rate of polysaccharides from the flowers, shoots and stems of Malus xiaoii, with the ratio of material to liquid, hot water soaking time at 80 ℃, ultrasonic time and ultrasonic power. Under the experimental conditions, the optimum conditions of polysaccharide extraction from different parts of Tamarix ramosissima were as follows: the ratio of material to liquid was 1:60, the time of soaking in hot water was 1h, the time of ultrasonic was 20min, and the ultrasonic power was 300W. At this time, the contents of polysaccharides in flowers, shoots and stems were 53.06,164.90 and 48.01 mg / g, respectively. The contents of polysaccharides in young shoots and leaves were significantly higher than those in the other two parts. The recovery rate of this method is 98.25%.