论文部分内容阅读
基于卡托普利对牛血红蛋白模拟酶催化体系的抑制作用,建立了酶催化光度法测定卡托普利的新方法。研究了该抑制反应的最佳实验条件及动力学行为,测定的线性范围为6.23×10-8～1.25×10-5 mol/L,检出限为3.88×10-9 mol/L。对浓度为6.23×10-6 mol/L的卡托普利进行11次平行测定,其相对标准偏差为3.5%,方法可用于卡托普利片中卡托普利含量的测定。
Based on the inhibitory effect of captopril on the catalytic system of bovine hemoglobin mimic enzyme, a new method for the determination of captopril by enzymatic spectrophotometry was established. The optimum conditions and kinetics of the inhibition reaction were studied. The linear range was 6.23 × 10-8 ~ 1.25 × 10-5 mol / L with the detection limit of 3.88 × 10-9 mol / L. The relative standard deviation of captopril in 6.23 × 10-6 mol / L was 11 parallel, and the relative standard deviation was 3.5%. The method can be used to determine the content of captopril in captopril tablets.