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目的:通过检测MCM7和HPV在上颌窦鳞癌(squamous cell carcinoma of the maxillary sinus,MSSCC)中的表达,探讨其在MSSCC发病机制中的作用及临床意义。方法:采用免疫组织化学方法检测MSSCC(实验组)、癌旁组织(癌旁组)和下鼻甲黏膜组织(对照组)中的MCM7及HPV的表达,应用SPSS 22.0软件包对数据进行统计学分析。结果:(1)MCM7在癌旁组和对照组上皮中大部分位于基底层,而其在MSSCC中呈弥散分布。MCM7在对照组(P=0.000)、癌旁组(P=0.000)和实验组(P=0.000)中的表达水平逐渐增高。(2)MCM7的表达仅与肿瘤分化程度有关(P=0.030),并且随着分化程度的下降,MCM7的表达水平逐渐增高。(3)伴HPV感染的细胞无论在对照组、癌旁组或实验组中分布均无明显规律。HPV在对照组、癌旁组及实验组患者中的感染率分别为40.00%(4/10)、95.65%(22/23)和78.57%(33/42)。(4)HPV感染与颈淋巴结转移有关(P=0.015),而与患者性别(P=1.000)、年龄(P=0.332)、吸烟(P=0.536)、临床分期(P=0.806)及肿瘤分化程度(P=0.489)无关。(5)在实验组中,伴HPV感染者,MCM7表达水平显著高于未感染者(P=0.007),且呈正相关关系(rs=0.317,P=0.041)。结论:MCM7和HPV可能与MSSCC的发生、发展有关,可能对患者的临床治疗及预后判断具有重要指导意义;MCM7异常表达可作为筛查HPV感染病例的良好临床病理学指标。
Objective: To investigate the role and clinical significance of MCM7 and HPV in the pathogenesis of MSSCC by detecting the expression of MCM7 and HPV in squamous cell carcinoma of the maxillary sinus (MSSCC). Methods: Immunohistochemistry was used to detect the expression of MCM7 and HPV in MSSCC (paracancer group), paracancer (paracancer) and inferior turbinate mucosa (control group). SPSS 22.0 software package was used to analyze the data . Results: (1) Most of MCM7 in the paracancer and control epithelium were located in the basal layer, while they were diffusely distributed in MSSCC. The expression of MCM7 in control group (P = 0.000), paracancer group (P = 0.000) and experimental group (P = 0.000) increased gradually. (2) The expression of MCM7 was only related to the degree of tumor differentiation (P = 0.030), and the expression level of MCM7 gradually increased with the degree of differentiation decreased. (3) There was no obvious regularity in the distribution of HPV infected cells in control group, adjacent cancer group or experimental group. The infection rates of HPV in the control group, paracancer group and experimental group were 40.00% (4/10), 95.65% (22/23) and 78.57% (33/42) respectively. (4) HPV infection was related to cervical lymph node metastasis (P = 0.015), but not related to patient’s gender (P = 1.000), age (P = 0.332), smoking (P = 0.536), clinical stage Degree (P = 0.489) has nothing to do. (5) In the experimental group, the expression of MCM7 in patients with HPV infection was significantly higher than that in non-infected patients (P = 0.007), and there was a positive correlation (rs = 0.317, P = 0.041). Conclusion: MCM7 and HPV may be related to the occurrence and development of MSSCC, and may play an important guiding role in the clinical treatment and prognosis of patients. Abnormal expression of MCM7 may be a good clinical and pathological index for screening HPV infection.