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目的 研究HPV18永生化人胚食管上皮细胞的细胞遗传学改变 ,为深入研究食管癌发病机理提供实验依据。 方法 采用Giemsa染色、G显带、间期核荧光原位杂交 (FISH)分析人胚食管上皮永生化细胞株 (SHEE)染色体数目异常和结构畸变。 结果 人SHEE第 10、2 0代细胞染色体众数分别为 5 8～ 6 3、5 7～ 6 4,第 6 1代为双众数 :5 8～ 6 0、6 3～ 6 5。G显带发现 ,在大多数核型中出现异常染色体 :1 del(1) (q12 ) ;2 .del(1) (p32 ) ;3.der(4 ) ,t(4 ;?)(q31;?) ;4.der(5 ) ,t(5 ;?) (q31;?)。FISH结果显示 ,1号 3体和 7号 4体伴随传代数目增加而明显增加。 8号 2体保持相对恒定。 结论 SHEE细胞伴随传代数目增加 ,染色体众数有双众数分布倾向 ,染色体数目异常表现为不平衡增加 ,结构异常的染色体恒定存在 ,提示细胞的永生化是一个由多染色体参与的多阶段过程。
Objective To study the cytogenetic changes of immortalized human embryo epithelial cells (HPV18) and provide experimental evidence for further study on the pathogenesis of esophageal cancer. Methods Giemsa staining, G - banding and interphase nuclear in situ hybridization (FISH) were used to analyze the chromosomal abnormalities and structural aberrations of human embryo esophageal epithelial immortalized cell line (SHEE). Results The chromosome numbers of the 10th and 20th generation of human SHEE were 58 ~ 6 and 3,57 ~ 6 4, respectively, and the 6th generation was double-doubled: 58 ~ 60 ~ 63 ~ 65. G-banding revealed abnormal chromosomes in most karyotypes: 1 del (1) (q12); 2.del (1) (p32); 3.der (4); t ?); 4.der (5), t (5;?) (Q31 ;?). The results of FISH showed that the numbers of body 1 and body 4 increased significantly with the passage number. 8 No. 2 body to maintain a relatively constant. Conclusion The number of SHEE cells increases with the number of passages, the number of chromosomes has the tendency of double-number distribution, the number of chromosomes shows unbalanced increase, and the chromosomes with abnormal structures persist, suggesting that the immortalization of cells is a multi-stage process involving multiple chromosomes.