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作者采用特殊的放射免疫测定法测定了子宫平滑肌瘤和瘤所在子宫肌层中的17β-雌二醇,雌酮和黄体酮的含量。方法;从患子宫平滑肌瘤接受子宫切除术的12例30岁到40岁的妇女获得子宫标本。在特定条件下将子宫内膜从子宫肌层挖去,并分离出平滑肌瘤。将肌层与瘤各取其有代表性的部份5gm,经过冷的0.9%氯化钠溶液,特制缓冲液处理,制成匀浆,再提取雌二醇、雌酮及黄体酮.使用特制抗血清对雌二醇、雌酮及黄体酮进行放射免疫测定(RIA)。结果发现平滑肌瘤中17β-雌二醇的含量明显高于子宫肌层中的含量特别是在增殖期。黄体酮的含量在二者中没有差别。雌酮的含量在子宫肌层中比在平滑肌瘤中要高,然而此差别没有统计学意义。黄体酮、17β-雌二醇及雌酮的含量在月经周期各期多少有所变化。但仅仅在平滑肌瘤组织中雌二醇的含量在增殖期比分泌期高。Spellacy 等证明在愚子宫平滑肌瘤的25例妇女中,雌二醇的血浆浓度与对照组没有差别,因此可以
The authors used a special radioimmunoassay to determine the levels of 17β-estradiol, estrone, and progesterone in the myometrium of uterine leiomyomas and tumors. METHODS: Uterine specimens were obtained from 12 women aged 30 to 40 years undergoing hysterectomy from uterine leiomyomas. The endometrium is extirpated from the myometrium under specific conditions and leiomyomas are isolated. The muscles and tumors were each taken as a representative part of 5gm, treated with a cold 0.9% sodium chloride solution, made into a special buffer, made into a homogenate, and then extracted with estradiol, estrone, and progesterone. Antiserum radioimmunoassay (RIA) of estradiol, estrone, and progesterone. The results showed that the content of 17β-estradiol in leiomyomas was significantly higher than that in the myometrium, especially in the proliferative phase. The progesterone content did not differ between the two. The level of estrone was higher in the myometrium than in the leiomyoma, however this difference was not statistically significant. The levels of progesterone, 17β-estradiol, and estrone vary somewhat during each period of the menstrual cycle. However, the estradiol content in leiomyoma tissue is higher in the proliferative phase than in the secretory phase. Spellacy et al. demonstrated that the plasma concentration of estradiol was not different from that of the control group in 25 women with uterine leiomyoma.