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目的探讨国内首次合成的放射性药物９９ｍＴｃＨＬ９１在脑血管疾病诊断中应用的可能性。方法对９９ｍＴｃＨＬ９１进行一般性质、标记率、体外稳定性、异常毒性和正常小鼠体内生物分布等实验。建立１５只大鼠脑缺血模型，并在该模型上进行９９ｍＴｃＨＬ９１体内分布和乏氧显像研究。结果①ＨＬ９１药盒标记简便，安全稳定。②正常小鼠静脉注射９９ｍＴｃＨＬ９１后血中放射性迅速下降，肝、肾和胃肠道的放射性较高，心肺清除很快，正常脑组织摄取低。③处死前脑部显像及处死后离体脑组织显像均于注射９９ｍＴｃＨＬ９１４小时后可见患侧脑组织较对侧放射性增高，且显示的缺血区范围大致相似。④在不同时相离体脑组织显像图上，利用感兴趣区技术，计算患／健侧大脑中动脉供血区脑组织放射性比值分别为０９８±００６、０９９±００５、１２９±００３、１５６±０１４、１６６±００６，除１与２、８与１２小时外，其余各组间两两比较差异均有显著性（Ｐ＜００５～Ｐ＜０００１）。⑤体外测量缺血侧脑组织与正常脑组织单位重量放射性比值随注射放射性药物的时间延长而逐渐增高。结论９９ｍＴｃＨＬ９１在缺血缺氧脑组织滞留增加，清除速度减缓。实验研究和临床应?
Objective To investigate the possibility of 99mTcHL91, the first radiopharmaceutical synthesized in China, for the diagnosis of cerebrovascular diseases. Methods 99mTc HL91 for general properties, labeling rate, in vitro stability, abnormal toxicity and normal mice biodistribution experiments. Fifteen rat models of cerebral ischemia were established, and 99mTcHL91 distribution in vivo and hypoxic imaging were studied in this model. Results ① HL91 kit marked simple, safe and stable. ② normal mice after intravenous injection of 99mTc HL91 blood radioactivity decreased rapidly, liver, kidney and gastrointestinal radioactivity, rapid cardiopulmonary clearance, normal brain tissue intake is low. ③ Before sacrifice brain imaging and brain tissue imaging after sacrifice were 99mTc HL 914 hours after injection, visible ipsilateral brain tissue increased radioactivity than the contralateral, and the extent of the ischemic area showed similar. (4) At the same time, the radioactive ratios of brain tissue in the contralateral middle cerebral artery were 098 ± 006,099 ± 0 05,129 ± 003,156 ± 014,166 ± 006, with the exception of 1 and 2,8 and 12 hours, the rest of the other groups were significant differences between the two groups ( P <005 ~ P <0001). ⑤ In vitro measurement of ischemic brain tissue and normal brain tissue unit weight radioactivity ratio with the injection of radiopharmaceuticals and gradually increased. Conclusion 99mTc HL91 in hypoxic-ischemic brain tissue retention increased, clearance rate slowed down. Experimental research and clinical should?