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目的观察沉默膜联蛋白Ⅱ(annexinⅡ,A2)基因对淋巴瘤Jurkat细胞凋亡的影响。方法采用A2 siRNA转染人淋巴瘤Jurkat细胞后应用RT-PCR和Western blot鉴定干扰效果。Annexin V-FITC/PI双染流式细胞术检测其对细胞凋亡的影响。结果Real-time PCR和Western blot检测结果表明,A2基因被成功抑制。流式细胞术检测得A2siRNA组细胞凋亡率为57.47%±2.10%,较阴性对照组(28.68%±1.21%)明显增加,差异有统计学意义(P<0.05)。结论A2基因可增强Jurkat细胞抗凋亡作用,A2 siRNA可诱导Jurkat细胞凋亡。
Objective To investigate the effect of silent annexin Ⅱ (A2) gene on the apoptosis of lymphoma Jurkat cells. Methods Transfection of human lymphoma Jurkat cells with A2 siRNA was used to identify the interference effect by RT-PCR and Western blot. Annexin V-FITC / PI double staining flow cytometry assay of apoptosis. Results Real-time PCR and Western blot results showed that A2 gene was successfully inhibited. The apoptosis rate of A2siRNA group was 57.47% ± 2.10%, which was significantly higher than that of the negative control group (28.68% ± 1.21%) by flow cytometry, the difference was statistically significant (P <0.05). Conclusion A2 gene can enhance the anti-apoptotic effect of Jurkat cells and A2 siRNA can induce Jurkat cell apoptosis.