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目的:分别采用MTT和ATPLiteTM-M发光法分析人B细胞刺激因子对培养的小鼠脾细胞的生物学活性。方法:分离小鼠脾细胞,培养72h后,MTT和ATP方法测定不同密度脾细胞的增殖,从中选定适当的脾细胞密度分析BLyS活性。然后,检测不同剂量的BLyS对脾细胞的刺激作用。结果:用MTT和ATPLiteTM-M发光法分析发现:BLyS在1、5、10和20mg/L时,都能明显促进小鼠脾细胞的增殖。结论:MTT法检测BLyS对小鼠脾细胞的增殖作用是一种简单、快捷的方法。利用这种细胞模型,可以对BLyS拮抗剂进行初步筛选以期获得具有潜在应用价值的治疗剂。
OBJECTIVE: To analyze the biological activity of human B cell stimulating factor on cultured mouse spleen cells by MTT and ATPLiteTM-M respectively. Methods: Mouse spleen cells were isolated and cultured for 72h. MTT and ATP were used to measure the proliferation of splenocytes of different density. The appropriate spleen cell density was selected to analyze BLyS activity. Then, spleen cells stimulated with different doses of BLyS were tested. Results: MTT assay and ATPLiteTM-M luminescence analysis showed that BLyS could significantly promote the proliferation of mouse spleen cells at 1, 5, 10 and 20 mg / L. Conclusion: MTT assay of BLyS on the proliferation of mouse spleen cells is a simple and efficient method. With this cellular model, BLyS antagonists can be screened for potential therapeutically useful therapeutics.