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目的:探讨温补肾阳方对创伤后应激障碍(posttraumatic stress disorder,PTSD)大鼠行为学及血浆17-OHCS、cAMP的影响。方法:选择体重和旷野试验(open-field test)评分相近的36只SD大鼠,随机分为空白组、PTSD模型组、温补肾阳组,应用分养和国际上已确认可作为制备PTSD动物模型的SPS法造大鼠PTSD模型,定期测定各组大鼠行为学,采用酶联接免疫吸附剂(ELISA)法检测血浆17-OHCS、cAMP含量,观察温补肾阳方对该模型大鼠的疗效。结果:PTSD模型组大鼠Open-Field评分、糖水摄取量、17-OHCS、cAMP明显低于空白组(P<0.05或P<0.01);治疗组可改善PTSD大鼠Open-Field评分、糖水摄取量、17-OHCS、cAMP,与模型组相比,有统计学差异(P<0.05)。结论:温补肾阳方对创伤后应激障碍的老鼠有改善作用。
Objective: To investigate the effects of Warming Kidney-Yang-Fang on behavior and plasma 17-OHCS and cAMP in posttraumatic stress disorder (PTSD) rats. Methods: Thirty-six SD rats with similar body weight and open-field test scores were randomly divided into blank group, PTSD model group, warm-tonifying and kidney-yang group, application and breeding and internationally recognized as preparation of PTSD animals The PTSD model was made by SPS method. The behavior of rats in each group was measured regularly. The plasma 17-OHCS and cAMP levels were measured by enzyme-linked immunosorbent assay (ELISA) . Results: Open-Field score, water intake, 17-OHCS and cAMP in PTSD model group were significantly lower than those in blank group (P <0.05 or P <0.01). The treatment group could improve Open-Field score, 17-OHCS, cAMP, compared with the model group, there was a significant difference (P <0.05). Conclusion: Wubu Shenyang Decoction can improve post-traumatic stress disorder in rats.