论文部分内容阅读
目的 2014年10月浙江省金华市荣光小学发生疑似病毒性脑膜炎暴发疫情,为及时查明病因,分析病原的分子特征并进行病原溯源。方法开展流行病学调查,采集患者脑脊液和咽拭子样本6份,检测人类肠道病毒(HEV)核酸和分离病毒,对HEV分离株VP1基因测序,进行同源性与进化分析。结果在全校328名学生中,发病7例,罹患率2.13%。患者年龄7~8岁;临床症状为发热、头痛、呕吐等;从5例患者6份样本中均检测到HEV核酸,其中3份脑脊液中分离到3株肠道病毒埃可30型(echovirus 30,E30);新分离株与E30原型株之间VP1区核苷酸和氨基酸同源性分别为81.8%和91.8%~92.1%;进化分析显示:新分离株位于E30 G基因亚型分支上,与2013年浙江省CHN/ZJ/RA-72/13株亲缘关系最近。结论引起2014年10月浙江省金华市荣光小学聚集性病毒性脑膜炎暴发疫情的病原为E30 G基因亚型病毒。
Objective In October 2014, an outbreak of suspected viral meningitis occurred in Rongguang Primary School, Jinhua City, Zhejiang Province. In order to identify the cause of the disease in time and analyze the molecular characteristics of the pathogen and trace the origin of the pathogen. Methods To carry out epidemiological investigation, 6 samples of cerebrospinal fluid (CSF) and throat swabs were collected, the human enterovirus (HEV) nucleic acids were isolated and the viruses were isolated. The VP1 gene of HEV isolates were sequenced and analyzed for homology and evolution. Results In the whole school 328 students, 7 cases of onset, the attack rate of 2.13%. The patients were aged 7 to 8 years old. The clinical symptoms were fever, headache and vomiting. HEV nucleic acids were detected in 6 samples from 5 patients. Among them, 3 strains of enterovirus 30 (echovirus 30 , E30). The nucleotide and amino acid homology of the VP1 region between the new isolate and the E30 prototype strain were 81.8% and 91.8% -92.1%, respectively. Phylogenetic analysis showed that the new isolate was located on the E30 G subtype branch, It has the closest genetic relationship with CHN / ZJ / RA-72/13 strain in Zhejiang Province in 2013. Conclusions The E30 G gene subtype virus that caused the outbreak of aggregated viral meningitis in Rongguang Primary School in Jinhua City, Zhejiang Province in October 2014 was etiological.