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近年来,由于水污染的加剧,病毒污染引起的水源性疾病正在增多,已经引起人民的广泛关注。即使很少量的病毒颗粒亦可致病。目前各种水样微生物学质量控制主要是通过常规监测粪大肠菌群和大肠埃希菌这样的细菌指示物来实现的,然而大多数观点表明环境水样中细菌和病毒污染水平并不相关,因而建立饮用水中病毒学监测指标十分必要。由于水样中只有少量病毒颗粒存在,进行水样检测前必须浓缩水样中的病毒。水中病毒的检测除了要有准确、灵敏的检测方法外,水中病毒的浓缩与回收的效率亦是决定病毒检测成败的关键。目前水样中病毒浓缩的方法很多,本文对常用的浓缩回收方法作一综述,并对各个浓缩回收方法的优缺点作一评价。
In recent years, due to the increase of water pollution, the waterborne diseases caused by virus pollution are increasing, which have aroused people’s attention. Even a small amount of virus particles can cause disease. At present, the quality control of all kinds of water microbiology is mainly achieved through routine monitoring of bacterial indicators such as fecal coliform and Escherichia coli. However, most opinions show that the level of bacterial and viral contamination in environmental water samples is not relevant, Therefore, the establishment of virological monitoring indicators in drinking water is necessary. Since only a small amount of virus particles in the water sample, water samples must be concentrated before the virus in the water sample. In addition to the detection of water viruses in addition to accurate and sensitive detection methods, the efficiency of water concentration and recovery of the virus is also the key to determining the success of the virus test. At present, there are many ways to concentrate the virus in water sample. In this paper, we review the commonly used methods of concentration and recovery, and make an evaluation of the advantages and disadvantages of each concentration and recovery method.