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目的:观察山茶籽油对去卵巢大鼠骨组织和骨代谢的影响,为山茶籽油防治绝经后骨质疏松症提供实验依据。方法:6月龄健康雌性SPF级SD大鼠80只,分成4个实验组(每组20只):①对照组、②去卵巢组、③去卵巢+己烯雌酚组(DTS)、④去卵巢+山茶籽油组(CSO)。治疗组用药:去卵巢+己烯雌酚组,皮下注射己烯雌酚,治疗剂量为300 ug/kg,2次/周,连续12周;去卵巢+山茶籽油组,采用经口灌胃方式进行用药(山茶籽油),治疗剂量为5 ml/kg,每天1次,连续12周。各实验组在第12周,麻醉大鼠后取左心血,用于测量血清雌二醇、黄体酮、促卵泡激素、黄体生成素及血清钙、磷和碱性磷酸酶;再将大鼠处死后,取股骨近端切片观察骨组织结构。数据进行统计学分析。结果:与对照组比较,去卵巢组的血清钙、磷和雌二醇明显降低(P﹤0.01),而血清碱性磷酸酶、促卵泡激素明显升高(P﹤0.01),骨组织切片呈骨质疏松的病理改变;与去卵巢组比较,2个治疗组的血清钙、磷和雌二醇明显升高(P﹤0.01),而血清碱性磷酸酶、促卵泡激素明显降低(P﹤0.01),骨组织切片骨质疏松的病理改变得到明显改善。结论:山茶籽油对去卵巢大鼠的骨质疏松症有治疗作用,与己烯雌酚治疗效果相近。
Objective: To observe the effects of camellia oil on bone tissue and bone metabolism in ovariectomized rats, and provide experimental evidence for the prevention and treatment of postmenopausal osteoporosis by camellia oil. Methods: Eighty healthy female SPF SD rats of 6 months old were divided into 4 experimental groups (20 in each group): ① control group, ② ovariectomized group, ③ ovariectomized group, DTS, ④ ovariectomized group, Camellia oil group (CSO). The treatment group: ovariectomized + diethylstilbestrol group, subcutaneous injection of diethylstilbestrol, a therapeutic dose of 300 ug / kg, 2 times / week for 12 weeks; ovariect + camellia oil group, the use of oral administration of gavage Oil) at a therapeutic dose of 5 ml / kg once daily for 12 weeks. At the 12th week of each experimental group, left ventricular blood was taken from anesthetized rats and used to measure serum estradiol, progesterone, follicle stimulating hormone, luteinizing hormone and serum calcium, phosphorus and alkaline phosphatase; then rats were sacrificed After the proximal femur section to observe the bone structure. Data for statistical analysis. Results: Compared with the control group, the levels of serum calcium, phosphorus and estradiol in the ovariectomized group were significantly decreased (P <0.01), while the levels of serum alkaline phosphatase and follicle stimulating hormone were significantly increased (P <0.01) Compared with the ovariectomized group, serum calcium, phosphorus and estradiol in the two treatment groups were significantly increased (P <0.01), while serum alkaline phosphatase and follicle-stimulating hormone were significantly decreased (P < 0.01). The histopathological changes of osteoporotic bone tissue were significantly improved. Conclusion: Camellia oil has a therapeutic effect on osteoporosis in ovariectomized rats, which is similar to that of diethylstilbestrol.