To investigate the association of serum levels of matrix metalloproteinase-12 (MMP-12) and a functional polymorphism in the promoter -82A/G of the MMP-12 gene with acute atherosclerotic cerebral infarction (ACI) .
MethodsAll 608 cases of acute ACI and 374 healthy controls were included in the study. Serum levels of MMP-12 were measured using the enzyme-linked immunosorbent assay (ELISA). At the same time, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on the -82A/G polymorphism in the MMP-12 gene.
ResultsThe serum levels of MMP-12 in ACI group ((17.36±9.12) ng/ml) were not significantly higher than those in the healthy control group ((17.42±7.70) ng/ml, t=0.047, P=0.962). The frequency of the AG+GG genotypes was not significantly different between the two groups (7.6% vs 5.9%, χ2=0.281, P=0.584), and the frequencies of the G allele were 3.8% and 2.9% in the ACI group and the control group respectively which were also not significantly different between the two groups (χ2=0.746, P=0.374).
ConclusionThere is no correlation between serum level with genetic polymorphism in MMP-12 promoter (-82A/G) and cerebral infarction.