论文部分内容阅读
目的制备CD11c单抗修饰的免疫脂质体并进行表征,验证其体外对树突状细胞(dendritic cells,DCs)的特异靶向性。方法采用薄膜分散法制备脂质体,通过化学交联法将CD11c单抗连接于脂质体上,利用高效液相法测定CD11c与脂质体的连接率,透射电镜观察脂质体形态,激光粒度测定仪测定粒径和Zeta电势,流式细胞仪检测脂质体上连接CD11c的活性和对DCs的靶向效果,激光共聚焦显微镜观察该免疫脂质体特异性靶向特点。结果成功制备了CD11c免疫脂质体,测得单抗连接率为(77.6±3.2)%,平均粒径为(151.2±1.6)nm,Zeta电位为(-32.0±1.8)mV。电镜下脂质体粒径均匀,表面光滑,多为圆形,少许椭圆形,无粘连。CD11c单抗连接于脂质体仍保持了良好的活性,体外表现出显著的DCs特异靶向性。结论制备的CD11c免疫脂质体对DCs细胞具有特异靶向性。
Objective To prepare and characterize the immunoliposome modified with CD11c monoclonal antibody to verify the specific targeting of dendritic cells (DCs) in vitro. Methods Liposomes were prepared by membrane dispersion method. CD11c mAb was linked to liposomes by chemical cross-linking method. The attachment rate of CD11c to liposomes was determined by HPLC. The morphology of liposomes was observed by transmission electron microscopy. The particle size and Zeta potential were measured by particle size analyzer. The activity of CD11c on liposomes and the targeting effect on DCs were detected by flow cytometry. The targeting characteristics of the immunoliposomes were observed by laser confocal microscopy. Results The CD11c immunoliposomes were successfully prepared and the conjugation rate was (77.6 ± 3.2)%. The average particle diameter was (151.2 ± 1.6) nm and the zeta potential was (-32.0 ± 1.8) mV. Electron microscopy liposome size uniform, smooth surface, mostly round, a little oval, no adhesion. CD11c mAb still retained good activity when it was linked to liposomes, showing significant DCs-specific targeting in vitro. Conclusion The prepared CD11c immunoliposomes have specific targeting to DCs.