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目的观察D-半乳糖致衰老模型大鼠学习记忆能力和行为学情况,并探讨中药的干预作用。方法大鼠每日一次颈背部皮下注射5%D-半乳糖100 mg/kg。诱导大鼠衰老模型,连续7周,观察衰老模型大鼠的自主活动次数、空间记忆能力、主动回避遭受电击能力、探究活动等行为学表现和学习记忆能力,并用抗衰老片与首乌延寿片进行干预,观察中药的干预作用。结果皮下注射D-半乳糖造模后,衰老模型大鼠自主活动次数显著减少(P<0.05,P<0.01),水迷宫试验探索路径长度和搜台潜伏期显著延长(P<0.05,P<0.01),旷场试验移动路程长度和直立次数显著减少(P<0.01),穿梭回避试验平均潜伏期、进入错误区时间显著增加(P<0.05,P<0.01)。给予抗衰老片与首乌延寿片干预后,衰老大鼠的自主活动次数显著增加(P<0.01),水迷宫试验探索路径长度和搜台潜伏期显著缩短(P<0.01),旷场试验移动路程长度和直立次数显著增加(P<0.01),穿梭回避试验平均潜伏期、进入错误区时间显著减少(P<0.05,P<0.01)。结论D-半乳糖致衰老模型大鼠的自主活动次数减少,对新环境探索能力下降,学习记忆力下降;抗衰老片与首乌延寿片等中药可有效增强衰老模型大鼠的行为活动,提高衰老模型大鼠的学习记忆能力。
Objective To observe the learning and memory abilities and behavior of aging rats induced by D-galactose and to explore the intervention effect of traditional Chinese medicine. Methods Rats were subcutaneously injected with 5% D-galactose 100 mg / kg once daily on the back of the neck. The model of aging was induced in rats for 7 consecutive weeks. The number of spontaneous activity, spatial memory, active avoidance of electric shock, explore behavioral performance and learning and memory abilities of aged rats were observed. Intervention, observe the intervention of traditional Chinese medicine. Results After subcutaneous injection of D-galactose, the number of spontaneous activity in aging model rats decreased significantly (P <0.05, P <0.01), and the path length and searching latency in water maze test significantly prolonged (P <0.05, P <0.01 ), The length of the open field test and the number of erections were significantly decreased (P <0.01). The average latency of shuttle avoidance test and the time of entering the wrong zone were significantly increased (P <0.05, P <0.01). The number of spontaneous activities in aging rats increased significantly (P <0.01), the path length of water maze test and search latency (P <0.01) were significantly shortened after the intervention of anti-aging tablets and Shouwu yanshou tablets. The moving distance (P <0.01). The average latency of shuttle avoidance test and the time of entering the error zone were significantly decreased (P <0.05, P <0.01). Conclusion D-galactose-induced senescence model of rats decreased the number of voluntary activities, to explore the new environment decreased ability to learn and memory decline; anti-aging tablets and Shouwu Yanshou Tablets and other traditional Chinese medicine can effectively enhance the behavioral activity of aging model rats, improve aging Learning and memory abilities of model rats.