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本文旨在探讨电刺激右侧尾壳核(caudate putamen nucleus,CPu)对双侧丘脑外侧背核(laterodorsal thalamic nucleus,LD)单个 神经元放电和海马(hippocampus,HPC)电图瞬时时间编码形式的调制性影响。用21只雄性Sprague-Dawley大鼠(150-250 g),重 复急性强直电刺激(60Hz,2s,0．4-0．6mA)右侧尾壳核(acute tetanization of the right caudate putamen nucleus,ATRC)诱发大鼠癫痫模 型,4通道同步记录双侧LD神经元单位放电和双侧HPC深部电图。结果如下:重复施加ATRC可以诱导大鼠出现(1)双侧 LD-HPC癫痫电网络间的功能性环状联系。起始点为对侧LD神经元原发性单位后放电,随后出现同侧LD神经元原发性单位 后放电,然后呈现同侧HPC电图原发性后放电,最终引起对侧HPC电图脱同步化效应;(2)双侧LD神经元放电脉冲间隔 (interspike intervals,ISIs)散点分布形式与刺激前呈现镜像对称特征。对侧LD神经元原发性后放电的ISI点分布基于底层而且持 续时间较长,具有更加明显的突触可塑性特征:(3)随着ATRC串次的增加,对侧LD神经元原发性单位后放电间的爆发式放 电时程逐渐延长、可以募集增强海马电图同步化电活动;显现对侧LD神经元单个放电脉冲与HPC电γ电振荡(20-25 Hz)间 的锁相(phase—lock)和锁时(time-lock)关系。结果提示:ATRC町以募集形成具有联系的双侧LD神经元放电和HPC电图特征性 的神经信息编码形式,以对侧更加明显。这些跨越大脑半球、涉及多结构的功能性神经信息网络的建立很可能是癫痫发生、 发展和扩布的重要信息编码机制。
This study aimed to investigate the effects of electrical stimulation of the right caudate nucleus caudate putamen nucleus (CPu) on single neuron discharge and hippocampal (HPC) electrocircuit transient time coding of bilateral dorsal hypothalamic nucleus (LD) Modulation effects. Twenty-one male Sprague-Dawley rats (150-250 g) were used for repeated acute tetanic stimulation of the right caudate putamen nucleus (ATRC, 60 Hz, 2 s, 0.4-0.6 mA) ) Induced rat epilepsy model, 4-channel simultaneous recording of bilateral LD neuron discharges and bilateral HPC deep electrograms. The results were as follows: Repeated application of ATRC induced a (1) functional circular association between bilateral LD-HPC epileptic networks in rats. The starting point for the contralateral LD neurons after the primary unit of discharge, followed by ipsilateral LD neuronal primary unit after discharge, and then showed ipsilateral HPC primary post-discharge, and eventually cause contralateral HPC electrogram Synchronization effect; (2) interspike intervals (ISIs) scatter distribution pattern of bilateral LD neurons showed mirror symmetry before stimulation. The ISI distributions of the contralateral LD neurons after primary post-discharge are based on the bottom layer and last longer, with more obvious features of synaptic plasticity: (3) With the increase of ATRC series, the contralateral LD neurons primary Unit discharge time between the explosive discharge gradually extended to raise the hippocampus synchronized electrical activity; show a contralateral LD neuron single discharge pulse and HPC electricity oscillation (20-25 Hz) between the phase-locked ( phase-lock and time-lock relationship. The results suggest that ATRC chooses to recruit encoded neuronal information that is characteristic of bilateral LD neuronal discharges and HPC electrograms that are associated with each other to be more evident on the contralateral side. The establishment of functional neural information networks spanning the hemisphere and involving multiple structures is likely to be an important information coding mechanism for the occurrence, development and spread of epilepsy.