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目的:研讨中药调肝理脾方和东宝肝泰片对乙醇性肝病肝纤维化的治疗效果。方法:♂SD大鼠经过9 wk乙醇混合物灌胃后,模型组被处死,取得肝脏,其他动物随机分成自然恢复组(11 wk)、东宝肝泰片组和调肝理脾方组,给予连续10 d的灌胃治疗,然后处死并取得肝脏,采用光镜、电镜观察肝细胞、肝星状细胞、肝窦内皮细胞、肝窦和窦周病理变化,并以免疫组化方法观察3种细胞外基质分子变化．结果:模型组动物肝细胞混浊肿胀,脂肪变性,间质细胞增生,细胞核深染,Masson染色并可见肝细胞周围铁丝网格样纤维化和中央静脉基底膜增厚;自然恢复组肝细胞病变有所好转,纤维化也有好转,调肝理脾方组好转最明显．电镜下显示,模型组肝窦基底膜增厚,窦周胶原原纤维大量增生,肝细胞微绒毛大量减少、倒伏,自然恢复组无明显好转,调肝理脾方和东宝肝泰片片组有好转．Ⅰ型胶原和层黏蛋白恢复结果以调肝理脾方组最好,Ⅳ型胶原恢复结果以东宝肝泰片组最好．结论:调肝理脾方对病理性堆积的Ⅰ型胶原和层黏蛋白代谢有明显促进作用,效果强于东宝肝泰片,而且也能促进过多的Ⅳ型胶原恢复正常,而东宝肝泰片在促进堆积的Ⅳ型胶原恢复方面强于调肝理脾方．
Objective: To study the therapeutic effects of Tiaogan Lipi Fang and Dongbaogantai tablets on hepatic fibrosis caused by alcoholic liver disease. METHODS: Rats in SD rats were intragastrically instilled with ethanol mixture for 9 weeks. The model group was sacrificed and liver was obtained. The other animals were randomly divided into natural recovery group (11 weeks), Dongbaogan Tablet group and Tiaogan Lipi Recipe group. After 10 days of continuous gavage, livers were sacrificed and livers were harvested. Pathological changes of hepatocytes, hepatic stellate cells, hepatic sinusoidal endothelial cells, hepatic sinusoids, and peri-sinusoids were observed by light and electron microscopy. Three types of immunohistochemical methods were used to observe the changes. Changes in extracellular matrix molecules. RESULTS: The hepatocytes of the model group were swollen with swelling, steatosis, hyperplasia of stroma cells, and deep staining of nucleus. Masson staining showed fibrosis of the hepatocytes around the wire mesh and thickening of the basement basal membrane. The hepatocyte lesions in the natural recovery group were slightly different. Fortunately, fibrosis also improved, the most obvious improvement in the liver and liver spleen group. Electron microscopy showed that the hepatic sinusoidal basement membrane thickened in the model group, with a large number of proliferative collagen fibrils around the sinuses, and the microvilli in the hepatocytes decreased significantly, while the natural recovery group showed no obvious improvement. The Tiaogan Lipifang and Dongbaogantai tablets group Have better. The results of type I collagen and laminin recovery were best in the Tiaogan Lipifang group, and the recovery results of type IV collagen were best in the Dongbaogantai tablet group. Conclusion: Tiaogan Lipi Recipe has obvious promoting effect on the pathological accumulation of type I collagen and laminin metabolism. The effect is stronger than Dongbaogantai tablets, and it can also promote the recovery of excessive type IV collagen, and Dongbao Liver Thai Tablets is more effective than the Tiaogan Lipi Recipe in promoting accumulation of type IV collagen.