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目的设计一种新型生物型人工肝,评价其治疗暴发性肝功能衰竭的疗效。方法采用无纺布编织型生物反应器接种原代猪肝细胞构建生物型人工肝,对D-氨基半乳糖胺诱导的猪暴发性肝功能衰竭模型进行治疗。结果生物型人工肝治疗可显著降低受试动物血氨和血清L-乳酸浓度,稳定血糖水平,改善Fischer指数,并延长动物存活时间。结论以无纺布编制型生物反应器为基础构建的生物型人工肝对暴发性肝功能衰竭具有明显的肝支持作用,具有临床应用可行性。
Objective To design a new type of bioartificial liver to evaluate its curative effect on fulminant hepatic failure. Methods A non-woven braided bioreactor was used to inoculate primary porcine hepatocytes to construct a bioartificial liver for the treatment of D-galactosamine induced porcine fulminant hepatic failure model. Results The bioartificial liver therapy could significantly decrease the blood ammonia and serum L-lactate concentration, stabilize the blood glucose level, improve the Fischer index, and prolong the survival time of animals. Conclusion The bioartificial liver constructed on the basis of non-woven weaved bioreactor has obvious hepatic support for fulminant hepatic failure, which has the feasibility of clinical application.