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F3-28是从杀虫植物杠柳Periploca sepium Bunge根皮中分离的一个具有杀虫活性的馏分,其主要成分为杠柳苷A、杠柳苷E和杠柳苷X。为了探索F3-28的初始作用部位,为深入研究其作用机理奠定基础,本研究采用经典的昆虫消化酶活性测定方法,比较了F3-28对小地老虎Agrotis ypsilon和东方粘虫Mythimna separata5龄幼虫消化酶系活性的影响。结果表明:对F3-28不敏感的小地老虎幼虫摄食F3-28后,其中肠蛋白酶、淀粉酶、脂肪酶的活性无显著变化。对F3-28敏感的东方粘虫幼虫摄食F3-28后2,4,8,10,12,24,48h,其中肠总蛋白酶活性分别为对照组的0.76,2.53,1.45,1.88,1.54,1.46,1.70倍,且和药物浓度呈依赖关系;类胰蛋白酶的活性分别为对照组的1.60,1.75,1.60,1.12,1.39,1.16,1.15倍(以BAPNA为底物)或1.68,1.95,1.53,1.26,1.15,1.13,1.14倍(以TAME为底物),且和药物浓度呈依赖关系;类胰凝乳蛋白酶活性分别为对照组的0.50,1.66,1.44,1.18,1.54,1.08和1.03倍,但和药物浓度无依赖关系;淀粉酶的活性分别为对照组的1.60,1.35,1.27,1.31,1.23和1.20倍,但和药物浓度无依赖关系;对脂肪酶活性无明显影响。这些结果说明,杠柳杀虫活性组分F3-28的作用机理可能涉及对试虫中肠蛋白酶的激活,特别是对类胰蛋白酶的激活。
F3-28 is an insecticidal active fraction isolated from the root bark of the insecticidal plant Periploca sepium Bunge. Its main constituents are Peripanil A, Peripanil E and Pergamon X. In order to explore the initial site of F3-28 and lay a foundation for further study of its mechanism of action, this study used classical methods for the determination of digestive enzyme activity in insects. F3-28 was used to compare the effects of F3-28 on Agrotis ypsilon and Mythimna separata 5th instar larvae Effect of digestive enzymes activity. The results showed that there was no significant change in intestinal protease, amylase and lipase activity in F3-28 insects that were insensitive to F3-28. The F3-28-sensitive Oriental armyworm larvae fed F3-28 2,4,8,10,12,24,48 h, the intestinal total protease activity of the control group were 0.76,2.53,1.45,1.88,1.54,1.46 , 1.70-fold, and the drug concentration was dependent; tryptase activity were 1.60,1.75,1.60,1.12,1.39,1.16,1.15 times (with BAPNA as a substrate) or 1.68,1.95,1.53, 1.26,1.15,1.13,1.14 times (TAME as a substrate), and the concentration dependence of the drug; chymase activity were 0.50,1.66,1.44,1.18,1.54,1.08 and 1.03 times the control group, But no correlation with drug concentration. The activity of amylase was 1.60, 1.35, 1.27, 1.31, 1.23 and 1.20 times higher than that of the control group, respectively. However, the activity of amylase was not dependent on the drug concentration. These results indicate that the mechanism of action of the F3-28 insecticidal active ingredient may involve the activation of enteroprotease in the test insects, especially tryptase.