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IL-17在儿童特异性过敏性疾病的发病中起着重要的作用,而Del-1(developmental endothelial locus-1)是最新发现的IL-17的负调节因子,本文通过观察Del-1在特异性过敏性疾病儿童体内的变化情况,探讨其对于儿童特异性过敏的临床价值。收集重度过敏组(n=18),轻中度过敏组(n=25)及对照组(n=23)的肺泡灌洗液(bronchoalveolar lavage fluid,BALF),用免疫荧光法检测BALF中的嗜酸性粒细胞阳离子蛋白(eosinophil cationic protein,ECP)水平,用RT-PCR的方法检测BALF的中IL-17和Del-1mRNA的表达。与对照组及轻中度过敏组比较,重度过敏组患儿BALF中的ECP及IL-17表达水平均明显升高(P<0.05);Del-1则明显下降(P<0.05),且Del-1与IL-17的表达水平呈直线负相关(r=-0.77,P<0.05)。炎症局部的Del-1表达水平与儿童过敏严重程度有重要的关系,并与IL-17相关,提示Del-1可能是小儿过敏性疾病中IL-17负调因子,研究结果可为有效治疗小儿过敏性疾病提供一个新的思路。
IL-17 plays an important role in the pathogenesis of childhood-specific allergic diseases. Del-1 (DEA) is the newly discovered negative regulator of IL-17. In this paper, To investigate the clinical value of childhood allergic diseases in children with allergic diseases. The bronchoalveolar lavage fluid (BALF) of severe allergic group (n = 18), mild to moderate allergic group (n = 25) and control group (n = 23) The levels of eosinophil cationic protein (ECP), IL-17 and Del-1mRNA in BALF were detected by RT-PCR. The levels of ECP and IL-17 in BALF in severe allergic group were significantly higher than those in control group and mild to moderate allergic group (P <0.05), while those in Del-1 group were significantly decreased (P <0.05) -1 was negatively correlated with the level of IL-17 (r = -0.77, P <0.05). The level of Del-1 expression in inflammation is related to the severity of allergic reaction in children and is associated with IL-17, suggesting that Del-1 may be a negative regulator of IL-17 in children with allergic diseases. The results of this study can be effective for children Allergic diseases provide a new way of thinking.